Yes, you can use the interactive mode to "tune-in" on an existing run and run commands on the latest files available. Put the following in a file:

package require openlane
prep -design <design_name> -tag <run_tag_name>; # this is the name of the directory containing the run files under designs/<design_name>/runs/<run_tag_name>

# if you want to change the DEF to be used by the next commands, then use:
# set_def /path/to/def/file

# your commands here:
run_magic

Then execute it using

./flow.tcl -interactive -file /path/to/above/script

@Ahmed Ghazy What is the TCL command used for setting the path of the SPICE file in case I want to just run LVS using netgen?

If you're on develop you can run

run_lvs /path/to/spice/netlist /path/to/verilog/netlist